Tối ưu hóa điều kiện trích ly flavonoids từ lá diếp cá

This study was conducted to investigate the application of cellulase

enzyme in the extraction of flavonoids from Houttuynia cordata

Thumb leaves and optimize the extraction conditions. Independent

variables, including enzyme concentration (25 - 100 µg/mL), temperature (30 - 50oC), time (30 - 120 min) and ratio of raw materials to

enzyme (1:15 - 1:30 g/mL), were investigated. Extraction conditions

of flavonoids were designed according to Central Composite Design

- Uniform Precision (CCD), a response surface methodology using a

software JMP Pro version 13. The results indicated that the optimal

extraction conditions were found to be enzyme concentration (78.0

µg/mL), temperature (41oC), time (90 min), and the ratio of

material to the enzyme (1:26 g/mL). Under such conditions, the

highest content of flavonoids (24.04 ± 0.05 mg/g, dry matter) was

obtained and validated. It can be concluded that the flavonoids can

be optimally extracted under the optimal extraction conditions with

assistance of cellulase.